Biology Reference
In-Depth Information
Chapter 53
Corner Turning Test for Evaluation of Asymmetry
After Intracerebral Hemorrhage in Rodents
Tim Lekic , William Rolland , Anatol Manaenko , Nancy Fathali ,
and John H. Zhang
Abstract
Intracerebral hemorrhage (ICH) is a relatively common and frequently fatal form of stroke that leads to
prolonged asymmetric sensorimotor defi cits in humans and experimental models. The test of corner turning
(CT) has been extensively used for long-term evaluation of sensorimotor damage in experimental models.
This is a quantifi cation of turning preference (right or left) upon approaching a 30° corner. Lesioned ani-
mals typically exhibit a turning preference which is highly correlated to the extent of striatal injury. This
test can stratify the amount of sensorimotor dysfunction between animals over time without the drawback
of learning after repeated testing. This chapter includes important descriptions required for successful
performance of this functional assessment tool.
Key words:
Behavior, Corner turn, Cerebral hemorrhage, Rats, Mice
1. Introduction
Intracerebral hemorrhage (ICH) is a fairly common and lethal
stroke subtype that leads to devastating sensorimotor defi cits in
surviving patients (
1, 2
). The secondary brain injury that follows in
the days after ICH, will exacerbate neurological disability even fur-
ther (
3
). There is a great need for potential therapeutics to be stud-
ied with the goal of improving functional outcomes for this patient
population (
4
). Striatal bleeding is the most common location for
humans, and has been represented with the rodent models of ICH,
which inject either autologous blood or collagenase into that brain
region (
5
).
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