Biochemical and Molecular Biological Assessments of Intracerebral Hemorrhage - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 634

Table 1
SDS-PAGE for BioRad MiniProtean gel

Stacking gel (4 mL) Separating gel (10 mL)

Acrylamide
concentration

5% 10% 12% 15%

30% Acrylamide mix 0.67 mL 1.7 mL 3.3 mL 4 mL 5 mL

1.5 M Tris, pH 8.8 2.5 mL 2.5 mL 2.5 mL 2.5 mL

1 M Tris, pH 6.8 0.5 mL

H 2 O 2.4 mL 5.7 mL 4.1 mL 3.4 mL 2.4 mL

10% SDS 40 mL 100 mL 100 mL 100 mL 100 mL

10% Ammonium
persulfate

30 mL 50 mL 50 mL 50 mL 50 mL

TEMED 3 mL 5 mL 5 mL 5 mL 5 mL

Fig. 1. Instruments and devices used for electrophoresis and Western blotting ( a ). Preparing gel for SDS-PAGE ( b ) and load-
ing of the samples into wells in the gel ( c ). The effectiveness of transfer of proteins from the gel to the membrane could be
checked by staining the membrane with Ponceau S dye ( d ). The molecular marker (M) is in the left line. The transferred
proteins were probed using antibody against hemoxygenase 1 (MW of HO-1 is ~32 kDa, e ) .