Biology Reference
In-Depth Information
Chapter 51
Biochemical and Molecular Biological Assessments
of Intracerebral Hemorrhage
Jin-Yul Lee , Ya Hua , Richard F. Keep , and Guohua Xi
Abstract
Spontaneous intracerebral hemorrhage (ICH) is a devastating condition associated with high morbidity
and mortality. Following the initial ictus, the intraparenchymal hematoma immediately triggers a series
of events leading to secondary brain injury and severe neurological defi cits. In an effort to understand
the underlying mechanisms of ICH-induced brain injury and to evaluate therapeutic interventions,
numerous experimental studies have been performed in our laboratories in last years, especially using
several biochemical and molecular biological methods. The most commonly employed techniques are
introduced below.
Key words: Intracerebral hemorrhage, Western blot analysis, Enzyme-linked immunosorbent assay,
Polymerase chain reaction
1. Western Blot
Analysis
Western blot analysis, fi rst described three decades ago by Towbin
et al. ( 1 ), is a powerful analytical method used to detect specifi c
proteins in a given sample of tissue homogenate. It has become an
essential technique for any biochemically oriented research labora-
tory. It uses gel electrophoresis to separate native or denatured
proteins by their molecular weight. The proteins are then trans-
ferred to a nitrocellulose membrane, where they are probed using
antibodies specifi c to the target protein ( 2 ). Described here is a
method to detect hemoxygenase (HO)-1 expression in brain tissue
following injection of autologous whole blood into the right basal
ganglia of the rat ( 3, 4 ).
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