Hemoglobin Measurements - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 631

1. This kit only detects rat Hb.
2. The results might be affected by the following factors: proper
instrument function, cleanliness of glassware, quality of dis-
tilled or deionized water, washing thoroughly and accuracy of
reagent and sample pipettings, washing technique, incubation
time, or temperature.
3. Neurons can produce own hemoglobin, which may affect the
measurement of hemorrhagic volume. Recent studies have
demonstrated that Hb is widely expressed in the brain and is
primarily neuronal ( 2-5 ). Our studies have also shown that Hb
is expressed in cerebral neurons and neuronal Hb is inducible
by cerebral ischemia ( 6 ).

2.6.2. Limitations

References

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