Biology Reference
In-Depth Information
Chapter 50
Hemoglobin Measurements
Yangdong He , Ya Hua , and Guohua Xi
Abstract
Hemoglobin (Hb) is an important oxygen transport protein mainly present in the red blood cells circulating
in the blood. Measuring brain Hb content has been used to assess hematoma mass after intracerebral
hemorrhage. Recent studies have demonstrated that Hb is also present in the neurons of embryonic and
adult brains. In this chapter, we introduce two methods for quantitation of Hb levels in the brain, includ-
ing spectrophotometric assay and enzyme-linked immunosorbent assay (ELISA). The details of the proce-
dures, along with results calculation, advantages, and limitations are discussed.
Key words:
Brain, Hemoglobin, Spectrophotometric assay, ELISA
1. Spectrophoto
metric Assay of Hb
The following instruments and materials for this assay are
recommended:
1.1. Materials
1. Electronic balance.
2. Homogenizer.
3. Spectrophotometer.
4. Cuvettes.
5. Microplate reader.
6. QuantiChrom™ Hb Assay Kit (BioAssay Systems). Reagent
and Calibrator are included in the kit.
7. Pipetting devices and Pipet Tips.
8. 0.1 mol/L PBS.
9. Tubes.
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