Immunological Response to Experimental Intracerebral Hemorrhage: Morphological Assessments - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 616

Fig. 5. Odyssey infrared scanner console. ( a ) The Odyssey Infrared Imaging System. ( b ) The LiCor scanner console. (1) To
scan microslides, leave the preset “membrane,” (2) The low-resolution 169 μ m is used to test scanning; normal scanning
resolution is 21 μ m. (3) “Medium” quality is acceptable only for test scanning. For real data acquisition, use the “highest”
quality, (4) Leave focus offset “0” mm, (5) Make sure the check boxes are selected for the dyes you want to scan. 700
corresponds to IRDye 700 reagents and 800 to IRDye 800 reagents. Set the “Intensity” for each channel. The signal inten-
sity for each channel should be determined at time of test scanning. Keep all scanning parameters consistent for all slides.
(6) The scan area. To reposition the scan area, click inside the red rectangle and drag the scan area to a new position.

6. Set the “Intensity” for each channel. The signal intensity for
each channel should be determined at the time of test scanning
(#5 in Fig. 5b ).
7. The scan area. To reposition the scan area, click inside the red
rectangle and drag the scan area to a new position (#6 in Fig. 5b ).

m) with “medium” quality
to get a quick preview test scan that allows reshape the scan area
and to optimize signal intensities (#2 and 3 in Fig. 5b ).
9. When the image area and the signal intensities are determined, use
the following scanning parameters: resolution: 21 μ m; quality:
highest, focus offset: “0” (#2-4 in Fig. 5b ).
10. It is important to keep the scanning parameters consistent
including the signal intensities for all image acquisitions. While
negative control sections have low signal intensities, they are
important for data correction (Table 6 , I-III).

8. Start a low-resolution scanning (169 μ