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Fig. 1. Arteriograph chamber for intact pressurized cerebral arteries. (
a
) Overhead view of
5 ml arteriograph chamber used for cannulation of cerebral artery segments. (
b
) Glass
micropipettes are pulled from capillary tubes and mounted in an arteriograph chamber.
(
c
) Image of a rat posterior cerebral artery (~150
μ
m diameter) cannulated on glass
micropipettes. Suture thread is used to tie each end of the artery segment onto the
micropipettes. (
d
) Image of rat parenchymal arteriole (~60
μ
m diameter). Video edge
detection (
black horizontal lines
) is used for measurement of arterial diameter.
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