The Stereology and 3D Volume Analyses in Nervous Tissue - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 33

Fig. 3. Microscopic photographs show that two groups of blood vessels supplying the
brainstem raphe reliably outline the dorsal and median raphe nuclei. A symmetrical contour
from the midline was drawn for the dorsal (DR, a ) and median raphe (MR, b ) ( arrowheads )
for stereological counting. Bar 150 m m. (From ref. ( 6 ) : Reprinted with permission from John
Wiley & Sons).
non-stereological methods (whether exhaustive or not), would
potentially require counting all signals in every section. This may
be impractical, as well as yielding incorrect results, where counted
objects cross between adjacent sections (even if only a subset of
sections were counted, the math extrapolating to the fi nal estimate
will overestimate by the same amount as if exhaustive cell profi le
counting was performed on all sections). Non-stereological sampling
in manual counting may introduce a number of potential selection
biases; for example, in a case where a user counts every fourth section