Electron Microscopic Assessment - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 467

4. Notes

1. Which fi xative is better?
Any aldehyde can be used for electron microscopy. In general,
glutaraldehyde and/or paraformaldehyde are more popular
than formaldehyde in electron microscopy. If the rest of the
specimen is used for immunostaining of light microscopy,
glutaraldehyde should not be used.
2. How to produce the specimen for scanning electron micros-
copy without injuring the luminal surface?
Like Fig. 2 , the end of the artery is stitched, only the adventitia
of the artery is picked up with microtweezers, and the artery is
cut with scissors under an operative microscope.

References

1. Endo S, Suzuki J (1979) Experimental cerebral
vasospasm after subarachnoid hemorrhage.
Participation of adrenergic nerves in cerebral
vessel wall. Stroke 10:703-711
2. Duckles SP, Bevan RD, Bevan JA (1976) An
in vitro study of prolonged vasospasm of a rabbit
cerebral artery. Stroke 7:174-178
3. Sönmez OF, Unal B, Inalöz S, Sahin B, Yilmaz
M, Aydin A, Kaplan S (2002) Therapeutic effects

of intracarotid infusion of spermine/nitric oxide
complex on cerebral vasospasm. Acta Neurochir
(Wien) 14:921-928
4. McGirt MJ, Lynch JR, Parra A, Sheng H,
Pearlstein RD, Laskowitz DT, Pelligrino DA,
Warner DS (2002) Simvastatin increases
endothelial nitric oxide synthase and ameliorates
cerebral vasospasm resulting from subarachnoid
hemorrhage. Stroke 33:2950-2956