Biology Reference
In-Depth Information
Chapter 37
Electron Microscopic Assessment
Norihito Shimamura , Akira Munakata , and Hiroki Ohkuma
Abstract
Electron microscopic changes of arterial wall during cerebral vasospasm have been eagerly examined in
1980s. Electron microscopy is still important assessment for investigating the etiology and pathogenesis of
cerebral vasospasm. The methodology has been established in spite of many variation, and standard materials.
Standard technique is introduced.
Key words: Transmission electron microscopy, Scanning electron microscopy, Paraformaldehyde,
Glutaraldehyde, Phosphate buffer, Cacodylate buffer
1. Introduction
Changes of ultrastructure in the arterial wall during cerebral vasos-
pasm have been evaluated under electron microscopy, and many
fi ndings have been reported. In addition to light microscopy,
electron microscopy is also fundamental assessment for cerebral
vasospasm, since the degree of arterial wall injury due to vasospasm
is evaluated by electron microscopy.
2. Materials
1. Fixative solution (see Note 1).
Several fi xative solutions in buffer have been used for electron
microscopy as follows:
(a) 2.5% Glutaraldehyde in 0.1 M sodium cacodylate buffer ( 1 ).
(b) 4% Paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) ( 2 ).
 
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