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Fig. 2. ( a ) HE staining of rabbit basilar artery. ( b ) Elastica van Gieson staining of canine
perforating artery at the Pons. ( c ) Immunostaining for endothelin-1 of canine basilar
artery.
a cryotome, and the section is dried, fi xed with acetone or 50%
ethyl alcohol, and washed with PBS. When the specimen is taken
after perfusion fi xation, it is usually embedded in paraffi n, sectioned
using a microtome, deparaffi nized with xylene, rehydrated with a
graded ethanol, and rinsed in PBS. After that, the process is same
as mentioned earlier.
If necessary (see Note 4), the section is incubated with 3%
hydrogen peroxide solution at room temperature for 5-10 min in
order to deactivate endogenous peroxidase. After being washed
with PBS, the section is incubated with blocking solution at room
temperature for 20-40 min in order to decrease background stain-
ing. And the section is incubated with a primary antibody at room
temperature.
After that, the process is divided according to ABC method,
LSAP method, and labeled polymer method (Fig. 4 ).
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