Biology Reference
In-Depth Information
Fig. 3. (
a
) Skin incision at the anterior chest. (
b
) Thoracotomy. (
c
) Pericardium is opened
(
d
) Ascending aorta (
open arrowhead
) is taken. (
e
) Left ventricle apex is cut and opened.
(
f
) Cannula (
open arrowhead
) is inserted and ligated at ascending aorta. (
g
) Right atrium
(
arrow head
) is cut and opened.
Second, the apex of left ventricle of heart is widely opened, and
cannula is upwardly inserted. Insertion of cannula into the ascend-
ing aorta is easily recognized by touch sensation of tip of cannula.
The ascending aorta is ligated with the thread (see the previous
step *1) together with the cannula inside it at just below the notch
of cannula.
Next, the descending aorta is cramped if you require it. Right
atrium is widely cut for draining the fi xative fl uid.
Perfusion is started with heparinized saline or Ringer solution.
The duration of those fl uids and/or their volume are varied accord-
ing to the literature and the kinds of animal. Usually, the duration
is between 10 and 25 min. Of course, draining fl uids from the
atrium becoming transparent is one of the indications of comple-
tion of this step.
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