Neurobehavioral Assessments of Subarachnoid Hemorrhage - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 416

Fig. 1. A handmade wire slope.

3. Methods

1. Each test is carried out prior to and after surgery at each inter-
val of 24 h until the sacrifi ce.
2. The examiner must have no knowledge of the procedure or
treatment that animals have undergone.
3. The neurobehavioral study consists of the following six tests
(Table 1 ).

1. Move an animal in the center of an empty animal cage.
2. Observe the animal's activity for 5 min.
3. The animal's activity is assessed by its ability to approach the
walls of the cage.
4. Scores indicate the following:
3, rat approaches and touches using both anterior limbs at least
three walls of the cage.
2, rat approaches and touches using both anterior limbs two walls
of the cage or reaches at least one upper rim of the cage.
1, rat approaches and touches using both anterior limbs one
wall of the cage.
0, rat cannot approach any wall of the cage.

3.1. Spontaneous
Activity