Biochemical and Molecular Biological Assessments of Subarachnoid Hemorrhage - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 408

instrument was introduced for proteomics analysis ( 32-34 ). This
system can obtain qualitative information from complex biological
samples ( 35-38 ). From these studies, it has been demonstrated
that nano-LC-ESI-MS when enhanced by the CHIP™ technology
has excellent resolution from complex systems, such as biologic
fl uids as well as good sensitivity and reproducibility. The newer
systems can perform a wide range of useful peptide identifi cation
routines for metalo proteins and phospho proteins using the public
access databases, such as Spectrum Mill (Agilent, Santa Clara, CA,
USA) and MASCOT (Matrix Science, London, England).
3. Ethos
For the ethos aspect of this argument concerning biochemical and
molecular assessments postsubarachnoid hemorrhage, we suggest
an examination of families of compounds to represent the events
involved. This will be useful for diagnosis as well as intervention
methodologies. We suggest six families of proteins that can be
expressed uniquely or frequently in the subarachnoid hemorrhage
patients and provide important information concerning vasospasm
and related complications. These families of molecules have been
chosen because they are consistent with the pathology in both cause
and effects of the disorder. Thus, their interpretation and informa-
tion contained therein can be prognostic for predicting future
events as well as diagnostic for assessing damage and pathology.
The families of protein markers will tell an interesting story
regarding the pathology and events seen postsubarachnoid hemor-
rhage during vasospasm. These are:
1. Mitochondrial and metabolic proteins,
2. Membrane, transmembrane, and membrane structural proteins,
3. Muscle and motility proteins,
4. Immune system and reactive oxygen species proteins,
5. Signaling cascade proteins and
6. Protein processing and pathology proteins.
All of these are relevant to neurovascular pathophysiology and
can tell us an enormous amount of important information con-
cerning the vasospasm patient and processes therein. For example,
an observation concerning muscle and motility proteins, such as
actin, myosin, actinin, and MLCK, are found in the CSF of vasos-
pasm patients suggests muscle dysfunction consistent with damaged
or pathologic arteries. When grouped into families, one or more of
these might be a marker for the process involved but patient-
to-patient variations would make it diffi cult to spot the disorder.