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Fig. 1. Dynamic imaging of BBB permeability in the rat neocortex (
a
and
b
). Averaged fl uorescent images from the surface
of the rat neocortex during tracer injection before (
a
) and after (
b
) perfusion with a solution containing high (K
+
) (30 mM).
Clear leakage of the fl uorescent dye is seen following treatment suggesting BBB breakdown. (
c
) Results of image analysis
are shown for maximal (max) value in each pixel. Note the robust increase in tracer intensity within the extravascular
region following treatment. (
d
) Intensity-time curves created for each compartment (i.e., arterial, venous, and extravascu-
lar) before (
black
) and after treatment (
red
) using cluster analysis revealed increase in incline and max parameters through
the vessels compartments, indicating for increase in fl ow. In the extravascular compartment, treatment with high (K
+
) led
to increase in max value following by slowing decay of the signal intensity, suggesting for accumulation of the tracer in this
compartment after BBB breakdown. (
e
) Injection of additional tracer with higher molecular weight, FITC-Albumin, and
detection of the signal behavior during the post-injection phase (
decline phase
) results with faster signal-decay of the NF
(
red
), suggesting for accumulation of the FITC-Albumin in the extravascular elements.
dynamic imaging method for quantifi cation of local changes in the
blood fl ow and permeability of small pial and surface cortical ves-
sels under physiological and pathological conditions (
26
). We
demonstrate the advantages and disadvantages of the approach and
present alternative established methodologies for the identifi cation
of BBB breakdown.
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