Aneurysmal Subarachnoid Hemorrhage Grading in Animal Models - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 310

Fig. 5. Simple regression analysis and Spearman's correlation coeffi cient by rank test was performed by Sugawara et al.
and found signifi cant correlation between the SAH grade and histological markers for vasospasm. Diameter ( r < −0.789,
p < 0.01), perimeter ( r < −0.86, p < 0.01) and wall thickness ( r > 0.901, p < 0.01). Figure adopted with modifi cation from
Sugawara et al. ( 11 ) .
Fig. 6. Simple regression analysis and Spearman's correlation coeffi cient by rank test was performed by Sugawara et al.
and found signifi cant correlation between the SAH grades of each investigator ( r = 0.937, p < 0.001). Figure adopted with
modifi cation from Sugawara et al. ( 11 ) .
5. Applications
and Limitations
This grading system was tested in the endovascular perforation
model for rats, but may be applicable in perforation models involv-
ing other species. However, this scale will probably not be as effec-
tive at quantifying hemorrhage if the site of perforation is not at
least within the Circle of Willis. In the endovascular perforation
model used to validate this model, perforation occurred at or near
the ICA bifurcation, which results in a substantial portion of the
blood remaining within the basilar cisterns. The distribution of
subarachnoid blood is not the same in all vascular puncture models,
such as basilar artery puncture, which has a paucity of blood near
the Circle of Willis ( 13 ). Its applicability in the use of injection
models may not be needed if the model itself has proven to consis-
tently provide stable blood clot sizes, such as the prechiasmatic