Acute Physiologic and Morphologic Assessment Following Subarachnoid Hemorrhage - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 292

Fig. 2. Cascade of injury following subarachnoid hemorrhage. Early brain injury results
from immediate physiologic disruption and mechanical damage to surrounding parenchyma.
Ultimate neuronal loss is consequence of multifactorial cascade leading to ischemia and
breakdown of cellular metabolism.

2. Methods

2.1. Animal
Preparation for
Neuropathology

Rodents are anesthetized with pentobarbital sodium (65 mg/kg,
i.p.) and perfused transcardially with 100 ml wash solution (0.8%
NaCl, 0.4% dextrose, 0.8% sucrose, 0.023% CaCl 2 , 0.025% sodium
cacodylate ) followed by 150 ml perfusion solution (4.0% sucrose,
4.0% paraformaldehyde, 1.072% sodium cacodylate). Brains are
removed from skull, stored in fi xative overnight, and incubated