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removed from the scanner, core temperature should be maintained
for the duration of recovery from anesthesia with a conventional
heating pad or heat lamp. For in vivo imaging experiments lasting
longer than a few hours, animals may need an injection of normal
saline to restore hydration at the conclusion of the study.
While the animal is out of sight inside the scanner, continuous
feedback on respiration rate and body temperature is essential for
maintaining appropriate anesthesia levels. Respiration sensors and
rectal temperature probes are available in MR-compatible versions
(e.g., SA Instruments Inc; CWE Inc). An MR-compatible pulse
oximeter (e.g., Starr Life Sciences) can also be used to monitor
blood oxygenation as well as heart rate and breathing rate during
scanning.
Although in vivo neuroimaging presents some unique experi-
mental challenges, it also offers enormous potential for elucidating
TBI pathology and developing novel treatments. Knowing these
potential challenges and designing your studies accordingly will
help to assure that reliable, high-quality imaging data is acquired.
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