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which is not subject to context or shock, had very low percentage
of freezing. Animals with contextual fear conditioning spent about
70% time on freezing. NNDA receptor inhibitor MK801 signifi -
cantly decreased freezing time, suggesting that MK801 impaired
the long-term memory in fear conditioning (
15
).
2.3. Note
Always use immediate shock for control. Animal is given electric
shock immediately after placing into chamber. In this situation,
animal does not show freezing in the context because it does not
have enough time to associate the context with the shock. This
control can eliminate the possible false-positive result, especially in
biochemical studies or genetic comparisons. It is very important to
score the freezing with the same criteria. The result of new experi-
menter should be compared with the experienced one by scoring
simultaneously. Grooming behavioral cannot count toward the
freezing. For new genotype animal in the laboratory, the baseline
freezing time should be compared with those published for that
genotype. The freezing time for auditory cued fear conditioning
is usually higher than that for the contextual fear conditioning.
The basic protocol takes 2 days. But be cautious that testing takes
longer time than training, so it is important not to train too many
animals in order to keep them in a controlled time frame.
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