Biology Reference
In-Depth Information
a
b
2000
Vehicle
Cocaine
Vehicle
Cocaine
800
1500
600
1000
400
500
200
0
0
1
2
3
4
5
6
1
2
3
4
5
6
30-min time blocks post-injection
30-min time blocks post-injection
c
d
Vehicle
SR 14150
2000
Vehicle
SR 14150
800
1500
600
1000
400
500
200
0
0
1
2
3
4
5
6
1
2
3
4
5
6
30-min time blocks post-injection
30-min time blocks post-injection
Fig. 3. (
a
) Cocaine (30 mg/kg, i.p.) increases distance traveled (cm) and rearing activity compared to vehicle baseline in
24-h recordings (
n
= 8 each group). (
b
) The nociceptin/orphanin FQ (N/OFQ) receptor (NOP), agonist SR 14150 initially
decreases distance traveled (cm) and rearing activity and later “rebound” hyperactivity (
n
= 4 each group) comparing to
vehicle baseline. Data were displayed between the injection time and 3 h following the injection for clarity.
by the SmartCage™. The nociceptin/orphanin FQ (N/OFQ) pep-
tide acting on its receptor (NOP) causes sedation, anxiolytic effect,
and modulates nociception (
22, 23
). These latter experiments were
conducted with SR14150 (
29
). SR14150 is an NOP receptor ago-
nist that is analgesic and produces reduced levels of activity relative
to controls when placed into an experimental setting (
23
). The same
procedure as described with cocaine was used whereby animals
received an injection of vehicle on the fi rst day and an injection of
the SR 14150 (10 mg/kg, i.p.) on the second day. SR 14150 pro-
duced an initial decrease in activity (e.g., distance traveled and rear-
ing) followed by a “rebound” and increase in activity (Fig.
3b
).
Search WWH ::
Custom Search