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own technical problems related to the specificity of the
substrate peptide, as well as degradation of the substrate by
nonspecifi c proteases, such as trypsin. With these assays,
additional controls must be done to confi rm that the fl uorescent
product is due to a specifi c metalloproteinase. Peptide assays
that use antibody capture method are more direct; however,
the total amount of gelatinolytic activity that can be extracted
from different brain regions may be limited and require that
samples are pooled.
4.6. Drug Manipulation
14. As with any pharmacological inhibitor, off target effects are
possible. This is always an issue with MMP inhibitors which,
given the high degree of homology among the MMP family
members, are not fully specifi c for individual MMPs. If such
drugs are used, these caveats must be considered with data
interpretation.
Acknowledgments
Studies are supported by funding from NIH NS044372 and
NS056247.
References
1. Ding JY, Kreipke CW, Schafer P, Schafer S,
Speirs SL, Rafols JA (2009) Synapse loss regu-
lated by matrix metalloproteinases in traumatic
brain injury is associated with hypoxia inducible
factor-1alpha expression. Brain Res 1268:
125-134
2. Grossetete M, Phelps J, Arko L, Yonas H,
Rosenberg GA (2009) Elevation of matrix met-
alloproteinases 3 and 9 in cerebrospinal fl uid
and blood in patients with severe traumatic
brain injury. Neurosurgery 65:702-708
3. Grossetete M, Rosenberg GA (2009) Bacterial
collagenase injection intracerebral hemorrhage
rat model. In: Chen J, Xu X-M, Xu ZC, Zhang
JH (eds) Animal models of acute neuro-
logical injuries. Humana Press, New York, NY,
pp 337-348
4. Homsi S, Federico F, Croci N, Palmier B,
Plotkine M, Marchand-Leroux C, Jafarian-
Tehrani M (2009) Minocycline effects cerebral
edema: relations with infl ammatory and oxidative
stress markers following traumatic brain injury
in mice. Brain Res 1291:122-132
5. Ranasinghe HS, Williams CE, Christophidis
LJ, Mitchell MD, Fraser M, Scheepens A
(2009) Proteolytic activity during cortical
development is distinct from that involved in
hypoxic ischemic injury. Neuroscience 158:
732-744
6. Reyes R, Guo M, Swann K, Shetgeri SU,
Sprague SM, Jimenez DF, Barone CM, Ding Y
(2009) Role of tumor necrosis factor-alpha and
matrix metalloproteinase-9 in blood brain barrier
disruption after peripheral thermal injury in
rats. J Neurosurg 110:1218-1226
7. Vilalta A, Sahuguillo J, Rosell A, Poca MA,
Riveiro M, Montaner J (2008) Moderate and
severe traumatic brain injury induce early over
expression of systemic and brain gelatinases.
Intensive Care Med 34:1384-1392
8. Yu F, Kamada H, Niizuma K, Endo H, Chan
PH (2008) Induction of MMP-9 expression
and endothelial injury by oxidative stress after
spinal cord injury. J Neurotrauma 25:184-195
9. Siffi nger M, Stefovska V, Zentner I, Hansen B,
Stepulak A, Knaute C, Marzahan J, Ikonomidou
C (2007) The role of matrix metalloproteinases
in infant traumatic brain injury. Neurobiol Dis
25:526-535
10. Yamaguchi M, Jaddhav V, Obenaus A, Colohan
A, Zhang JH (2007) Matrix metalloproteinase
inhibition attenuates brain edema in an
in vivo
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