MicroRNA Expression Profi ling of Rat Blood and Brain Tissues: TaqMan Real-Time PCR MicroRNA Assays - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 160

Fig. 2. Loading the cards into the swing bucket adapters of the centrifuge.

Fig. 3. Sealing the microfl uidics card after centrifugation.

3.7. Statistical
Analysis

1. RQ Manager is used to view results of the assay. The cycle
threshold (CT) is the primary measure used to determine the
original abundance of the specifi ed miRNA in the sample. The
CT is defi ned as the fractional cycle number at which the fl uo-
rescence passes the fi xed threshold. CT scores greater than 35
are considered nonspecifi c (undetectable) ( 8 ), so miRNAs in
which 95% of individual observations had a raw CT score
greater than 35 are excluded from the fi nal data analysis.
2. Average CT scores should be obtained and exported from RQ
Manager as a .txt fi le for import into other programs (e.g.,
Partek Genomics Suite or Genespring GX) for statistical analyses