MicroRNA Expression Profi ling of Rat Blood and Brain Tissues: TaqMan Real-Time PCR MicroRNA Assays - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 159

Fig. 1. Dispensing the PCR reaction mix into the port of the TaqMan miRNA fl uidic card.

6. Centrifuge twice at 1,200 rpm (331 ×g) for 1 min with maximum
acceleration and deceleration (Fig. 2 ), seal the card (Fig. 3 ),
and then trim the card (Fig. 4 ).
7. Import the appropriate SDS setup fi le (SDS.txt) located on the
Information CD (supplied with the TaqMan miRNA fl uidic
card):
(a) Start the SDS 2.3 software.
(b) In the main menu, select File → New .
(c) In the New Document dialog box, select the following
from the drop-down menu:

Relative Quantifi cation ( DD Ct) .

●

● 384-well TaqMan Low Density Array .
(d) In the main menu, select File → Import to open the new
document.
(e) In the Open dialog box, navigate to the Setup.txt fi le spe-
cifi c for the array being run and click Import .
8. Load the card into the 7900HT (Fig. 5 ) and run using the
384-well TaqMan Low Density Array default thermocycling
conditions (10 min at 95°C, followed by 40 cycles of 95°C for
15 s and 60°C for 1 min).