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Fig. 2. Cutting neocortical slices. After the brain is removed from the skull and placed on a piece of fi lter paper two parallel
coronal cuts are made as indicated by the dotted lines ( a ). The brain is then glued onto the slicing stage, with the caudal
surface on bottom (cutting surface of line 2) and the dorsal side facing the blade ( arrow ). When a brain from a young rat is
cut, a small block of agar ( dotted line rectangular ) should be glued onto the stage to support the brain ( b ). The brain is cut
with a Vibratome ( c ), and slices are transferred to an incubation chamber maintained in an incubator at 32°C for 1 h ( d ) .
Fig. 3. Field potential recording setup. ( a ) A pipette puller is used to make recording electrode from glass capillaries. ( b ) On
top of an interface chamber (1) is a cortical slice ( arrow ), which is in contact with a stimulating electrode (2) and a recording
glass electrode (3). Both of them are attached to micromanipulators (4).
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