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Fig. 2. Cutting neocortical slices. After the brain is removed from the skull and placed on a piece of fi lter paper two parallel
coronal cuts are made as indicated by the
dotted lines
(
a
). The brain is then glued onto the slicing stage, with the caudal
surface on bottom (cutting surface of line 2) and the dorsal side facing the blade (
arrow
). When a brain from a young rat is
cut, a small block of agar (
dotted line rectangular
) should be glued onto the stage to support the brain (
b
). The brain is cut
with a Vibratome (
c
), and slices are transferred to an incubation chamber maintained in an incubator at 32°C for 1 h (
d
) .
Fig. 3. Field potential recording setup. (
a
) A pipette puller is used to make recording electrode from glass capillaries. (
b
) On
top of an interface chamber (1) is a cortical slice (
arrow
), which is in contact with a stimulating electrode (2) and a recording
glass electrode (3). Both of them are attached to micromanipulators (4).
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