Biology Reference
In-Depth Information
Fig. 6. Schematic drawing of a simple brain slice incubator.
Grants Pass, OR), microscope translation stage (MXMS-100,
SD Instruments), infrared differential interference contrast
(IR-DIC) microscope plus 40× water immersion objective
(BX51WI, Olympus Optical, Tokyo, Japan), video camera
(OLY-150IR/DIC, Olympus America, Center Valley, PA), and
video monitor (PVM-137, Sony, Tokyo, Japan). Use of micro-
scope translation stage allows the movement of microscope in
searching the cells for recording (Fig. 7 ). The stage holding
the brain slice is fi xed. Such setup provides the opportunity for
double-electrode recording on the same slice.
2. Electrode puller (P-97, Sutter Instruments, Novato, CA).
3. Borosilicate glass (TW150-3, World Precision Instruments,
Sarasota, FL).
4. Bipolar tungsten stimulus electrodb (5 M , MicroProbes,
Potomac, MD).
5. Extracellular solution perfusion system (Valve Driver II, Parker
Hannifi n Corporation, Cleveland, OH).
6. Eight channel perfusion valve control system (VC-8, Warner
Instruments, Hamden, CT).
7. Automatic temperature controller (TC-324B, Warner
Instruments).
8. Device for securing slice in recording chamber (Fig. 8 ). The
U-shaped frame is made with a piece of tungsten wire. Then,
three to fi ve single strands (paralleled with 0.3-1.0 mm apart)
of nylon stocking are tightly stretched and glued across the
two arms of the frame.
9. Computer and data-acquisition program (AxoGraph or
pCLAMP software, Molecular Devices).
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