EEG, Evoked Potential, and Extracellular Single-Unit Recordings In Vivo - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 90

Fig. 2. Representative of an atereotaxic frame (Model SR-6 M, Narashige, Japan, Fig. 2-1),
a homeothermic blanket control unit and heating pad from Harvard Apparatus (Fig. 2-2),
and an automatic syringe pump from Harvard Apparatus (Fig. 2-3).

2.1.5. Data Acquisition and
Analysis Software

For EP experiments, we use pClampex 9.2 for data acquisition and
pClampfi t 9.2 for data analysis for our PC. For single-unit recording,
we use software package from Sky-A4 Bioelectric signal processing
system Mathlib (Fudan University; Shanghai, China).

2.2. Other Equipment

We use vibration isolation tables (Fig. 1b -6, c-8) and a Faraday
cage (Fig. 1b -5, c-7) from TMC for both EP and single-unit
recording.

2.2.1. Vibration Isolated
Table and Faraday Cage

2.2.2. Electrode Puller

We use PP-830 vertical electrode puller (Narashige, Japan).

We use Model SR-6M (Narashige, Japan, Fig. 2 -1).

2.2.3. Stereotaxic Frame

We use Narashige P-5N Motion controller (Fig. 1c -4).

2.2.4. Electrode
Manipulator

Our homeothermic blanket control unit and heating pad are from
Harvard Apparatus (Fig. 2 -2).

2.2.5. Heater

2.3. Anesthesia

EEG . (Rodents): 87 mg/kg ketamine plus 13 mg/kg xylazine (i.p.)
EP . (Cat): -chloralose 60 mg/kg (i.v.)

Single-unit recording . (Rodents): Chloral hydrate (400 mg/kg, i.p.)

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