Calcium Phosphate and Calcium Phosphosilicate Mediated Drug Delivery and Imaging - Intracellular Delivery: Fundamentals and Applications - page 738

Fig. 10 Bovine aortic endothelial cells treated with free Cy3 or Cy3 encapsulated in calcium
phosphosilicate nanocomposite particles dosed with cytochalasin-D to inhibit endosome
formation . Bovine aortic endothelial cells were stained using Cy3 dye, either as free dye ( a , b ) or
as dye encapsulated in calcium phosphosilicate nanoparticles ( c , d ). Cell in panels ( b , d ) have
been treated with cytochalasin-D to inhibit formation of endosomes. Arrows indicate staining of
organelles which does not occur for cells treated with cytochalasin-D and incubated with Cy3
indicating that endosomal capture with pH changes as shown in Fig. 2 are required to dissolve the
calcium phosphosilicate particles to release the encapsulated Cy3 (Reproduced from Morgan
( 2008 ). With permission)
window and fluoresce bright enough for deep tissue imaging (Altınoğlu 2008 ).
In vivo images of these particles show successful targeting to both breast and
pancreatic cancers in nude mice (Altınoğlu 2008 ; Barth 2010 ). With a PEGylated
surface, ICG-CPSNPs circulate for over 96 h (Fig. 11 ) and accumulate in breast
tumors via the enhanced permeation and retention effect (Altınoğlu 2008 ;
Morgan 2008 ). In addition, covalently bound antibodies on the PEG surface,
target specific cancers, such as pancreatic cancer and show increased localization