Membrane Crossover by Cell-Penetrating Peptides: Kinetics and Mechanisms – From Model to Cell Membrane Perturbation by Permeant Peptides - Intracellular Delivery: Fundamentals and Applications - page 179

Membrane Crossover by Cell-Penetrating
Peptides: Kinetics and Mechanisms - From
Model to Cell Membrane Perturbation
by Permeant Peptides

Isabel D. Alves, Nicolas Rodriguez, Sophie Cribier, and Sandrine Sagan

Abstract Membrane-active peptides are a large family endowed with a wide pattern
of biological activities (antimicrobial, viral fusion and infection, cell-penetrating or
protein-transduction domain), which share the property of interacting with mem-
branes and being internalized in eukaryotic cells. Apart from pinocytosis internaliza-
tion pathways, these peptides have the capacity to re-organize lipid membranes and
to lead to membrane fusion, disruption or pore formation. In this chapter, we focus
on these membrane perturbation processes evoked by cell-penetrating peptides that
have been widely studied with membrane models and in cultured cells.

Keywords Cell-penetrating peptide • Membrane • Permeation • Pinocytosis
• Translocation

Abbreviations

AMP Antimicrobial Peptide

Antp antennapedia, homeoprotein

CHO chinese hamster ovary cells

CPP Cell-Penetrating Peptide

CS Chondroitin Sulphate

DOPC dioleoylphosphatidylcholine

DOPG dioleoylphosphatidylglycerol

DPPC dipalmitoyl phosphatidylcholine

DSC Differential Scanning Calorimetry

ESR Electron Spin Resonance spectroscopy

GUV Giant Unilamellar Vesicle

I.D. Alves, N. Rodriguez, S. Cribier, and S. Sagan ( * )
Laboratory of Biomolecule, Universite Pierre et Marie Curie, Paris,
75252 Cedex 05, France
e-mail: [e-mail]