Biology Reference
In-Depth Information
free energy binding of S to E* and thus able to do work either internally
(e.g., modulation of the rate of electronic transition) or externally on enzyme's
environment as in myosin head exerting a force on the actin filament (see
Sect.
11.4
).
5. The transition from
a
to
c
(without being mediated by state
b
) is what is involved
in the
Circe mechanism of enzymatic catalysis
as proposed by Jencks (1975).
Since this mechanism is not based on the generalized Franck-Condon principle,
the Circe effect mechanism may be viewed as theoretically incomplete.
In Sect.
11.3
, PMR as stated in Statements 3.9 and 3.10 and GFCP will be
applied to elucidate the molecular mechanisms underlying the action of cholesterol
oxidase based on the single-molecule fluorescence measurements made in (Lu et al.
1998).
