High-Throughput Analysis of Microdissected Tissue Samples - High-Throughput Image Reconstruction and Analysis - page 244

Figure 11.1 LCM of an intraepithelial lesion of the uterine endocervix (endocervical glandular
dysplasia). Dysplastic epithelium (arrows) was specifically procured from a tissue section. (a) Tissue
before dissection. (b) Tissue after dissection. (c) LCM cap image showing the dissected cells. 10 × ,
Hematoxylin and Eosin stain.
11.2.4.3 Ultraviolet (UV) Laser--Based Systems
Ultraviolet-based dissections systems have also been developed, including the laser
microdissection system and pressure catapulting technology (P.A.L.M. MicroLaser
Figure 11.2 Schematic image showing laser-based microdissection methods, including LCM,
PALM, and the LMD cutting system. (a) A transparent LCM cap containing a transfer film is placed
on top of the tissue under microscopic visualization. The laser beam is directed through the cap
to all sites in the field containing desired cells, activating the film. The cap is then lifted, procuring
all targeted cells. (b) A UV laser is used to generates photonic pressure and catapult cells into a
microcentrifuge tube after UV cutting. (c) Desired cell areas are cut by a UV laser and fall by gravity
into a collection tube.