Agriculture Reference
In-Depth Information
Fig. 5.10.
The molecular identification of viruses in 12 randomly selected garlic
plants from a field in Aomori province, Japan. The photographs show bands on
electrophoresis gels from Polymerase Chain Reaction (PCR) amplifications of DNA
sequences characteristic of four different garlic viruses: GV1 carlavirus, GV2
potyvirus, OYDV onion yellow dwarf virus and mite-borne virus. The numbered
columns show each of the 12 plants tested; columns N and P, respectively, are
negative and positive controls for the diagnostic DNA bands, and column M gives a
sequence of markers of increasing numbers of DNA base-pairs. The chart below the
photographs indicates which viruses tested positive for each plant and, on the last
line, which plants displayed visual symptoms of viral disease (from Takaichi
et al
.,
1998. Courtesy of
Plant Disease
).
