Biomedical Engineering Reference
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Fig. 11.2 Process chart for
togwa preparation
as they hydrolysed starch [ 14 ] . The dominance of L. plantarum was probably
because of its acid tolerance and its ability to effectively utilise different carbon
sources. Obligative heterofermentative Lactobacillus brevis, L. fermentum and
W. confusa , and homofermentative Pediococcus pentosaceus were also among
dominant LAB strains in togwa [ 14 ] . Issatchenkia orientalis was the predominant
yeast whereas S. cerevisiae, Candida pelliculosa and C. tropicalis were also among
the most common yeast isolates (Table 11.2 ).
Hellström et al. [ 16 ] isolated a number of yeast strains from togwa fermenta-
tions, identified them using API and molecular techniques, and determined their
phytase activity. Yeast strains with high phytase activity could serve as alternatives
for mineral fortification or the addition of phytases in order to improve the mineral
availability of fermented foods through elimination of the metal-chelating com-
pound phytate. The lactic acid bacteria may also promote phytate elimination as the
acidification increases the activity of endogenous cereal phytases [ 17 ] .
In addition to the elimination of antinutritive compounds, the microflora of togwa
may produce beneficial compounds such as B vitamins in food fermentations.
Hjortmo et al. [ 18 ] investigated folate production in togwa fermented with previously
isolated togwa-adapted yeasts [ 16 ] . Tetrahydrofolate and 5-methyl-tetrahydrofolate
were the dominant forms. The concentration of the latter folate type substantially
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