Use of Indigenous Bacteria from Arsenic Contaminated Soil for Arsenic Bioremediation - Management of Natural Resources in a Changing Environment - page 158

Table 5 Growth response versus as remov al by KUMAs1 under aerobic culture condition

Growth and As removal
As(III) As(V)

Incubation Growth a % removal Growth % removal

48 9.19 54.67 10.24 56.13

72 8.93 51.22 9.09 50.95

96 7.51 35.34 7.95 37.89

a Growth responses are presented as log number of CFU/ml
All the treatment regimes were inoculated with same inoculum load as stated

Conclusions

It appears that strain KUMAs1 is effective in removing As under aerobic conditions,
though it showed As(V) reducing ability. Furthermore, this activity is maintained
even at low concentrations of As. Based on the present study, it seems that KUMAs1
could be used as potential As bioremediating candidate. Being an indigenous iso-
late, KUMAs1 might be assumed to have better rhizosphere colonizing ability in the
paddy fi eld; additional feature of this isolate makes it important that it could pro-
duce IAA, thus it might be used as biofertilizer candidate as well. Though, its effi -
ciency under environmental condition conducive for decontamination of arsenic
requires further investigation.

Acknowledgements This work was supported by the fi nancial grants received from DST, Govt.
of India under INSPIRE Programme and DBT, Govt. of India.

References

Ahmann D, Roberts AL, Krumholz LR, Morel FMM (1994) Microbe grows by reducing arsenic.
Nature 371(6500):750
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J
Mol Biol 215(3):403-410
Anderson CR, Cook GM (2004) Isolation and characterization of arsenate-reducing bacteria from
arsenic-contaminated sites in New Zealand. Curr Microbiol 48:341-347
ASA (1982) Methods of soil analysis. Part 2. Chemical and microbiological properties, 2nd edn.
Page AL (ed). Agronomy Society of America, Madison
Bachate SP, Cavalca L, Andreoni V (2009) Arsenic-resistant bacteria isolated from agricultural soils
of Bangladesh and characterization of arsenate-reducing strains. J Appl Microbiol 107:145-156
Banerjee S, Datta S, Chattyopadhyay D, Sarkar P (2011) Arsenic accumulating and transforming
bacteria isolated from contaminated soil for potential use in bioremediation. J Environ Sci
Health A Tox Hazard Subst Environ Eng 46:1736-1747
Bhattacharya P, Samal AC, Majumdar J, Santra SC (2009) Transfer of arsenic from groundwater
and paddy soil to rice plant ( Oryza sativa L.): a micro level study in West Bengal, India. World
J Agric Sci 5(4):425-431