Environmental Engineering Reference
In-Depth Information
Typically iron nanoparticles are synthesized either by aqueous routes using
co-precipitation of iron (II and III) salts (Bandyopadhyay et al.
2011
) or by high
temperature route using toxic iron pentacarbonyl compounds. Further purifi cation
of these particles is needed and surface modifi cations of these are also a laborious
task. Here we demonstrate a low cost novel synthetic method for the preparation of
a stable suspension of iron nanoparticles. One-pot synthesized mono-dispersed iron
nanoparticles were characterized using UV-vis Spectrophotometer, DLS, TEM,
SEM and its bio-compatibility has been checked on bacterial viability. The as-
synthesized iron nanoparticles were then utilized for Cr(VI) and MG remediation
from contaminated water.
Methodology
Reagents and Materials
All the chemicals and reagents used in this work were of analytical grade and pur-
chased from Merck India. Double distilled water (DDW) made at our laboratory
was used throughout the study. The stock solutions of Chromium (VI) (2.0
g/mL)
and of MG (50 mg/mL) was prepared in DDW and experimental solutions of their
desired concentrations were obtained by successive dilutions of the stock solution
with DDW. For measurement of Cr(VI) and Malachite Green (MG) DDW was arti-
fi cially contaminated and was used for all the experiments.
ʼ
Instrumentation
A dual beam Techcomp UV2300 UV-vis spectrophotometer was used for determin-
ing concentrations of Cr(VI) and MG in the water samples. Size distribution and
Zeta potential (
) of the MNPs were characterized using transmission electron
microscopy (TEM; Tecnai S-Twin, FEI, USA), scanning electron microscopy
(SEM; S-2300, Hitachi, Japan) and dynamic light scattering (Zetasizer, Malvern
Instruments Ltd, UK).
ʶ
Bacterial Culture
Gram-negative
E. coli DH5ʱ
and gram-positive
B. subtilis
were obtained from
Department of Molecular Biology and Biotechnology, University of Kalyani. The
bacteria were maintained and incubated in shaking incubators, using Luria-Bertani
(LB) medium at a temperature of 37 °C.
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