Chemistry Reference
In-Depth Information
Analyte
t
R
, min
Quantitation ion, m/z
Triadimenol
7.66
296.1
Cyproconazole
8.55
292.1
Myclobutanil
10.08
289.1
Triadimefon
10.48
294.1
Tebuconazole
10.96
308.1
Hexaconazole
11.85
314.1
Fenbuconazole
12.25
337.1
Propiconazole
12.82
342.1
Difenoconazole
13.14
406.2
t
R =
Retention time
Table 1.
List of Triazole, their retention time and Quantitation ions
Figure 4.
Mass spectra of triazoles; Fenbuconazole (337.1), Difenoconazole (406.1), Myclobutanil (289.1),
Tebuconazole (308.1), Propiconazole (342.1), Triadimefon (294.1), Triadimenol (296.1), Hexaconazole
(314.1) and Cyproconazole (292.1 ).
3.2.1. Mass spectrometer tuning
Before the chromatographic method was established, the mass spectrometer was tuned to
optimize the conditions of parameter for both the formation and detection of ions during an
analysis. It is also done to increase the sensitivity and to optimize the mass peak resolution
for the application. Optimization of both the ionization process and ion transportation in the
mass spectrometer is important to achieve high sensitivity and selectivity and low detection
limits in liquid chromatography / atmospheric pressure chemical ionization spectrometry
(LC/APCI-MS) analysis. The optimization was done by changing one-variable-at-a time
while the others are kept constant.
The mass spectrometer tuning was done using two methods; by infusing a sample with the
syringe pump and also from the syringe pump into the LC flow line. This is to see the effect
of mobile phase flow rate and composition on signal intensity and to allow optimization of
the source parameters without making numerous injections in order to achieve parameters
giving the highest sensitivity. Infusion experiments were carried out to examine the
ionization and fragmentation patterns of the analytes. The instrument parameter; corona
voltage, cone voltage, desolvation flow and temperature, cone flow and mass resolution
