Homocysteine-Thiolactone - Homocysteine in Protein Structure/Function and Human Disease - page 37

Fig. 3.7 Hcy-thiolactone and N-Hcy-protein formation in cultured human endothelial cells.
Confluent cultures of human umbilical vein cells are labeled with [ 35 S]Hcy (330 to 5,000 Ci mol 1 )
for 48 h. Panels (a) and (b): Levels of N-Hcy- protein (filled square) and protein-Met (filled circle)
in intracellular (a) and extracellular (b) proteins as a function of [ 35 S]Hcy concentration. Panels (c)
and (d): levels of Hcy-thiolactone as a function of [ 35 S]Hcy (c) and Met (d). Panels (e) and (f):
Autoradiograms of two-dimensional TLC separations of [ 35 S]amino acids liberated by acid