Living on Acetylene. A Primordial Energy Source - The Metal-Driven Biogeochemistry of Gaseous Compounds in the Environment - page 33

and the results from site-directed mutagenesis. In the case of a first shell mecha-
nism, the more polar reaction product, acetaldehyde will have to pass through a
narrow hydrophobic gate upon leaving the active site, energetically not a favorable
event. On the other hand, in a second shell mechanism, acetaldehyde would just be
expelled into the substrate channel, assisting the product release by the repellant
interaction of a polar molecule with a hydrophobic surrounding [ 27 ].

Abbreviations and Definitions

10 10 m

Å Å ngstr¨m; 1 ż

acetyl-CoA acetyl-coenzyme A

ADP adenosine 5'-diphosphate

AH acetylene hydratase

AH(Mo) acetylene hydratase, molybdenum-substituted

AH(W) acetylene hydratase, in its native tungsten form

ATP adenosine 5'-triphosphate

CD circular dichroism

1 g · mol 1
DFT density functional theory
DMSO dimethyl sulfoxide
DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen
dtc dithiocarbamate
dtl dithiolate
EPR electron paramagnetic resonance
HEPES 2-[4-(2-hydroxyethyl)piperazine-1-yl]ethanesulfonic acid
ICP-MS inductively coupled plasma-mass spectrometry
MALDI-TOF matrix-assisted laser desorption/ionization time of flight analysis
MGD

Da Dalton; 1 Da ¼

molybdopterin-guanine-dinucleotide

MPD 2-methyl-2,4-pentanediol

NAD + /NADH ʲ -nicotinamide adenine dinucleotide (oxidized/reduced)

NarG nitrate reductase G subunit from E. coli

NarG-AH acetylene hydratase with a fused chaperone binding sequence

from NarG

PDB Protein Data Bank

PEG polyethylene glycol

SDS-PAGE sodium-dodecyl sulfate polyacrylamide gel electrophoresis

min 1

U unit; 1 U ¼ 1 ʼ mol

·

UV/Vis ultraviolet/visible

v/v volume per volume