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of reusable modules, in line with the biobricks of iGEM. The basic modules can be
well characterized and then assembled in different ways to obtain the desired
circuit. The development of computer-aided design (CAD) tools will be necessary
to allow assembly and in silico testing of the new circuit. These CAD tools should
not only help in vector and chromosome construction (as is generally the case
today), but also include functions for predicting fluxes and help in designing the
optimal regulatory interactions. Once assembled in the organism, high throughput
screening methods will have to be used to fine-tune and debug the de novo designed
system. No fundamental obstacles separate us from this future.
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