MicroRNAs and Robustness in Biological Regulatory Networks. A Generic Approach with Applications at Different Levels: Physiologic, Metabolic, and Genetic - Systems Biology of Metabolic and Signaling Networks - page 91

Table 4.11 Number of common subsequences of length 6 and 7 between the mitochondrial tRNA
loops and the noncoding mitochondrial genomes of capra (total length 1,531 bases) and canis (total
length 3,239 bases) ( http://megasunbchumontrealca/ogmp/projects/other/cp_list html)
Subsequences Observed capra Expected capra Observed canis Expected canis

TACCAC 1 0.4 0 0.8

TACCAT 0 0.4 0 0.8

ACCGTT 1 0.4 0 0.8

ACCATT 1 0.4 0 0.8

CTTGAA 0 0.4 0 0.8

ATTTGAA 0 0.1 0 0.2

Total 3 2.1 2.9 0 4.2 4.1

Table 4.12 Number of common subsequences of length 6 and 7 between the mitochondrial tRNA
loops and the noncoding mitochondrial genomes of ruminants (total length 10,024 bases) and
nonruminants (total length 7,774 bases) ( http://megasunbchumontrealca/ogmp/projects/other/
cp_list html). The stars correspond to the statistical significance of the difference between the
ruminants and non ruminants totals of matches

Observed
ruminants

Expected
ruminants

Observed
nonruminants

Expected
nonruminants Subsequences

TACCAC 3 2.4 0 1.9

TACCAT 1 2.4 0 1.9

ACCGTT 3 2.4 0 1.9

ACCATT 2 2.4 0 1.9

CTTGAA 1 2.4 0 1.9

ATTTGAA 1 0.6 0 0.5

Total 11** 12.7 7.1 0** 10 6.3

**p < 0.01.

animals like horse (equus) and dog (canis) have an efficacy in O 2 consumption
about 2.5 times more than ruminants like steer (bos) and goat (capra). We have then
studied in the noncoding (called d-loop) mitochondrial genome of the nonruminants
if there are less mitomiRs candidates than in those of ruminants.
Table 4.10 shows that such mitomiRs exist and contain long subsequences
(whose length is between 6 and 7 bases) common with the mitochondrial tRNA
loops. On the contrary, these subsequences are totally absent in the noncoding
mitochondrial genome of the nonruminants. These genomes have been extracted
for these different animals from the classical genetic databases dedicated to the
whole mitochondrial ( http://megasunbchumontrealca/ogmp/projects/other/cp_list.
html ) and chloroplast ( http://www.ncbinlmnihgov/nuccore/EF115542 ; http://www.
ncbinlmnihgov/nuccore/AB042240 ) genomes: Megasun, from the University of
Montreal ( http://megasunbchumontrealca/ogmp/projects/other/cp_list.html ) , and
Nuccore, from the NCBI ( http://www.ncbinlmnihgov/nuccore/EF115542 ; http://
www.ncbinlmnihgov/nuccore/AB042240 ) .
From Tables 4.10 , 4.11 , and 4.12 , we see that the absence of mitochondrial small
subsequences, we have called mito 2 miRs, in the noncoding (called d-loop) mito-
chondrial genomes of nonruminants (for a total length of 10,024 bases) susceptible
to hybridize the mitochondrial tRNAs inside the mitochondrion matrix is highly