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passage 30.]
[They were routinely grown at 37°C in Dulbecco's modified
Eagle's medium (DMEM) containing 10 % fetal bovine serum
(FBS), 2 mM glutamine, 1 mM sodium pyruvate, and 25 mM
glucose (Invitrogen, Carlsbad, CA, USA) in a humidified
atmosphere containing 5% CO2.]
[For precursor and 13C-sugar experiments, tissue culture
treated polystyrene 35 mm dishes (Corning Inc, Lowell, MA,
USA) were seeded with 2 × 106 cells and grown to confluency
in DMEM.]
When executed against medical text, this model will perform better than other models.
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