EXPERIMENTAL APPROACHES TO THE STUDY OF DRUG TRANSPORTERS - Drug Transporters: Molecular Characterization and Role in Drug Disposition - page 533

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EXPERIMENTAL APPROACHES
TO THE STUDY OF DRUG
TRANSPORTERS

YOSHIYUKI KUBO, YUKIO KATO, AND AKIRA TSUJI
Kanazawa University, Kakuma, Kanazawa, Japan

18.1. Introduction
18.2. In Vivo Experiments
18.2.1. Preparation of Knockout Mice
18.2.2. Preparation of Knockdown Mice
18.2.3. Comparative Study of Normal and Knockout or Knockdown Mice
18.3. Isolated Tissue Methods
18.3.1. Loop Method
18.3.2. Ussing-Type Chamber Method
18.3.3. Everted Sac Method
18.3.4. Sliced Organs
18.4. Primary Cell Cultures and Established Model Cell Lines
18.4.1. Isolated Hepatocytes
18.4.2. Fibroblasts
18.4.3. Established Cell Lines
18.4.4. Transfected Cell Lines and Xenopus laevis Oocytes
18.4.5. Techniques to Study Cellular Uptake or Transport
18.5. Membrane Vesicles
18.5.1. Membrane Vesicles from Cultured Cells
18.5.2. Membrane Vesicles from Tissues
18.5.3. Rapid Filtration Technique