Biomedical Engineering Reference
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FIGURE 8.2.
Secondary structures predicted for hCNT1 (
a
) and hENT1 (
b
). The N-
glycosylation sites are indicated as “Y.” Transmembrane domains important for substrate-
inhibitor interactions are shaded in gray. Residues shown as solid circles have been implicated
in interaction with substrates or inhibitors.
possess 13 transmembrane (TM)
-helices (Figure 8.2
a
). A CNT1 ortholog has also
been cloned from pig.
11
In humans, hCNT1 mRNA is expressed predominantly in the liver and kidney and
exhibits much lower expression in other tissues.
12
There appear to be large interindi-
vidual differences of hCNT1 mRNA expression level in the kidney.
12
In rat, rCNT1
mRNA was found in the jejunum, kidney, liver, and various regions of the brain.
13
,
14
Western blot further demonstrated the expression of rCNT1 protein in the small in-
testine, kidney, and liver.
15
Fluorescence protein-tagged constructs of hCNT1 and
rCNT1 were expressed on the apical membranes of polarized LLC-PK1 and MDCK
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