Ballast Water Sampling and Sample Analysis for Compliance Control - Global Maritime Transport and Ballast Water Management: Issues and Solutions - page 186

Table 4 Possible sampling access points, equipment and other details recommended for indicative
in-line compliance control sampling with the D-2 standard (David 2013 )
Organism
group

Sampling
point

Water volume
[litre] Equipment Number of samples

>50 ʼ m In-line Plankton

net

300-500 in
each sequence

1 sequential sample of ca. 10 min
duration, avoiding the very
beginning and very end of the
tank discharge event

<50 and
>10 ʼ m

In-line Bucket 5-6 1 continuous drip sequential

sample, may be simultaneously
collected during sampling of
organism group > 50 ʼ m

Indicator
microbes

In-line Bucket 1 1 continuous drip sequential

samples, may be sub-sampled
from the bucket

Description of Sampling Methods for Indicative In-Line D-2 Sampling

We recommend that for an in-line indicative ballast water sampling event, one
sequential sample is taken using the same methodology as for a detailed D-2 stan-
dard compliance test (see sections “ Detailed In-Line D-2 Standard Sampling ” and
“ Recommendations for a Ballast Water Sampling Protocol that Is Representative of
the Whole Discharge ”).

Detailed Sampling for Compliance Control
with the D-2 Standard

Detailed In-Tank D-2 Standard Sampling

Because D-2 is a discharge standard, in-tank sampling for a detailed analysis is of lim-
ited value. However, in cases when in-tank sampling reveals very high organism num-
bers, non-compliance may be assumed also when this ballast water is discharged. To
illustrate this, should a sample from the tank contain 1,000 viable organisms greater
than or equal to 50

m in minimum dimension and the tank capacity is 100 t, the organ-
ism concentration would exceed the D-2 standard when the water is discharged.

ʼ

Detailed In-Line D-2 Standard Sampling

Selection of Sampling Equipment and Methods for Detailed In-Line D-2
Standard Sampling

Our previous on board ballast water sampling studies on commercial vessels have
shown that different sampling approaches, i.e., short/long sampling times, result in dif-
ferent concentrations of viable organism (Gollasch and David 2009 , 2010a , b , 2013 ).