Biomedical Engineering Reference
In-Depth Information
Fig. 10.7
Overview
of chromatin
immunoprecipitation
methodology
10.9.3
Chromatin Immunoprecipitation
Chromatin immunoprecipitation (ChIP) is a powerful procedure for analyzing
epigenetic modifi cations and to identify genomic DNA sequences bound to specifi c
regulatory proteins, such as HAT proteins (Fig.
10.7
). First, living cells or tissues
are fi xed with formaldehyde forming a strong cross-link between histones and
genomic DNA. After shearing and solubilizing by sonication, pulldown is per-
formed using an antibody for acetylated histones or proteins associated with
