Bacterial Adhesion and Interaction with Biomaterial Surfaces - Biointerfaces: Where Material Meets Biology - page 381

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Figure 13.7 Bacterial adhesion on smooth and textured 500/500 polyurethane sur-
faces (a) S. epidermidis RP62A in PBS for 1 h, (b) S. epidermidis RP62A in
25% human plasma for 2 h, (c) S. aureus Newman in PBS for 1 h, and (d)
S. aureus Newman in 25% human serum for 2 h. *P o 0.05, **P o 0.01,
and ***P o 0.001.
Reproduced from Xu and Siedlecki 153 with permission from Elsevier.

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Figure 13.8 Fluorescence microscopy images of S. aureus Newman biofilms formed
on (a) smooth and (b) textured 500/500 polyurethane surfaces after 5
days incubation in 25% serum and 25% brain heart infusion solution at
a shear stress of 2.2 dyn cm 2 (scale bar: 100 mm).
Reproduced from Xu and Siedlecki 153 with permission from Elsevier.

Reduction in bacterial adhesion on a textured surface leads to inhibition
of biofilm formation. Both S. epidermidis and S. aureus formed a biofilm and
produced slime on smooth control sample surfaces, while only individual
bacteria were seen on a textured surface. Figure 13.8 shows an example of a