Bacterial Adhesion and Interaction with Biomaterial Surfaces - Biointerfaces: Where Material Meets Biology - page 374

0.8 2000

1600

0.6

1200

d n 3 r 4 n g | 3

0.4

* *

# # #

***

800

0.2

400

* **

*

0 0

Polyurethane surfaces with protein pre-adsorption

Fn recognition Fn_C-terminus

Fn_N-terminus bacteria adhesion

Figure 13.2 Correlation of molecular Fn recognition, and amino (N)- or carboxy (C)
termini of Fn, and bacterial adhesion on polyurethane surfaces. Stat-
istical analysis symbol # denotes the comparison of Fn recognition to
surfaces adsorbed from pure Fn (0.01 mg mL 1 ) solution, and * denotes
comparing N- and C-termini on same sample.
Reproduced from Xu and Siedlecki 128 with permission from Scientific
Research Publishing.

.

microscopy) to assess the Fn adsorption/orientation on polyurethane sur-
faces at the molecular scale. The orientation of Fn adsorbed on polyurethane
surface was detected by the monoclonal antibodies (MAb) coupled AFM
probes. Results show that Fn adsorbed on polyurethane surfaces from pure
Fn solutions showed more C-terminus available than N-terminus in same
sample, and corresponded to higher bacterial adhesion (Figure 13.2), indi-
cating that S. epidermidis bacterial cell surface prefers to bind at the domain
of C-terminus of Fn. Furthermore, the presence of albumin influences the
orientation of Fn and more N-terminus is available although the amount of
Fn adsorption is lower in this case. 128

13.3.3 Interaction of Bacteria and Platelets on Biomaterial
Surfaces
Pathogenic bacteria can occasionally enter the human circulatory system
and lead to the interaction of bacterial pathogens with platelets, resulting in
life-threatening diseases such as stroke. Interaction of bacteria and platelets