Biomedical Engineering Reference
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d
n
3
r
4
n
g
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3
Figure 12.11 Plot of percent adhering number of HUVECs and relative surface
density of the cell-binding motifs on fibronectin.
Reproduced/adapted from ref. 44 with permission from Taylor &
Francis.
.
materials surfaces that easily induce fibrinogen adsorption also induce ad-
sorption of other cell adhesive proteins such as fibronectin and vitronectin
at the same time on its surfaces.
45
The reason why the selective cellular re-
sponses could be achieved on the dynamic PRX surface could be explained
in the point of view of the different adsorption states of proteins. In the case
of adsorbed fibrinogen on the mPRX surface, the specific binding site for
platelet GPIIb/IIIa was eliminated in much lower level than Cell Desk
t
even
though the amount of adsorbed proteins was much higher level than Cell
Desk
t
. However, the cell-binding motifs of the surface fibronectin on the
mPRX revealed almost similar level to Cell Desk
t
. This suggests that the
modulated conformational change of the cell adhesive proteins in serum is
one of the reasons why the mPRX surface showed moderate fibroblast
adhesion as eliminating platelet adhesion.
Of particular interest in Figure 12.11 is the different morphology of ad-
hering HUVECs on various polymer surfaces having different value of Mf.
Because the morphology of adhering cells is determined by the continuous
communication between cells and the microenvironment of the adhering
surfaces,
46
this result suggests the possibility that dynamic nature of the
polymer surfaces make an effect on not only the adsorption states of pro-
teins but also the adhering morphology of cells.
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