Biomedical Engineering Reference
In-Depth Information
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Figure 10.3 Examples of high-throughput methods for the optimisation on cellular
response: (A) encoded microparticles, (i) schematic of synthesised
particles, (ii) fluorescence image of DNA oligomer loaded particles,
after incubation in fluorescently labelled targets; (B) microfluidic de-
vices, (i) schematic of microfluidic device, (ii) confocal microscopy
images showing effect of neuron repellent precursors on neuron re-
sponse; (C) protein/cell microarrays, (i) overview of entire array showing
many different polymer concentrations and compositions, (ii) human
mesenchymal stem cells grown on polymer arrays, (iii) close up of
individual spots; (D) gradient surfaces, for example plasma polymer
gradients of acrylic acid to octadiene, (i) XPS % COOH content and
photo showing the cell attachment of mouse embryonic stem cells
across the AA-OD gradient, (ii) bright field images showing alkaline
phosphatase stained colonies, (iii) phase contrast images showing
different colony morphologies.
Figures A, B, C and D adapted from Pregibon et al.,
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Kothapalli et al.,
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Anderson et al.
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and Wells et al.,
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respectively.
.
time, allows for many different polymers, polymer compositions, chemical
functionalities or biomolecules to be investigated on a single substrate.
75,79
Hence, thousands of samples to be screened without any knowledge or ex-
pectation for the outcome, with spots indicating an interesting response to
be investigated further in greater detail. For example, Anderson et al.
80
have
investigated the effect of polymer composition on the behaviour of human
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