Protein-based Biointerfaces to Control Stem Cell Differentiation - Biointerfaces: Where Material Meets Biology - page 3

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CHAPTER 1
Protein-based Biointerfaces to
Control Stem Cell
Differentiation

JORGE ALFREDO UQUILLAS PAREDES, ALESSANDRO POLINI
AND WOJCIECH CHRZANOWSKI*

Faculty of Pharmacy, University of Sydney, Sydney, Australia
*Email: [e-mail]

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1.1 Modification of Biomaterial Surfaces with
Proteins
It is already well-established that cellular responses to foreign materials
when implanted in vivo are guided mainly by the surface characteristics. The
modulation of characteristics such as roughness, porosity, chemical and
biological composition allows the regulation of material integration within
the body as well as the guidance of specific responses, e.g., cell adhesion, cell
detachment, cell proliferation, differentiation, or metabolic activity. Material
integration within the body is critically important for orthopedic implan-
table devices. Desired integration results in successful single surgery, thus
reduces costs and complications related to adverse reactions and implant
revisions. In this chapter, we described the methods of surface modification
and functionalization, which enable the effective regulation of stem cells
behavior for orthopedic applications. In particular, the chapter focuses on
immobilization of different signaling molecules such as proteins, peptides,
growth factors on the surface and, in three dimensions, to modulate cell