Titanium Phosphate Glass Microspheres as Microcarriers for In Vitro Bone Cell Tissue Engineering - Biointerfaces: Where Material Meets Biology - page 129

present is to use the microspheres as substrates in perfusion bioreactors.
These bioreactors could potentially allow the development of small-sized
tissue fragments that would be useful for tissue replacement strategies.
A major indicator of the success of these microspheres is whether the results
obtained for MG63 cells can be replicated with stem cells, which offer re-
markable possibilities for guided differentiation through particular lineages.
However, the major challenges encountered in carrying out these cell culture
approaches using stem cells are: (1) the significantly weaker attachment
between the cells and the microsphere surface, and (2) the slow growth rates
associated with these cells. It is anticipated that these challenges can be
overcome by suitable biofunctionalisation of the microsphere surface and by
the use of cell culture media that are more suited to rapid stem cell pro-
liferation. Thus, the development of viable small-sized bone tissue to treat
bone loss or bone defects caused by injury, disease or congenital deformities
is now an achievable objective.

d n 3 r 4 n g | 3

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